abstract：:Enzymatic markers and electron microscopy were utilized to determine the cellular origin of the membrane types isolated from type 2 dengue virus-infected BHK cells by discontinuous sucrose gradient centrifugation. The results showed an apparent separation of plasma membrane, smooth and rough endoplasmic reticulum with...

journal_title：Journal of virology

authors： Stohlman SA,Wisseman CL Jr,Eylar OR,Silverman DJ

更新日期：1975-10-01 00:00:00

abstract：:Extracellular murine leukemia virus (MLV) reverse transcriptase activity was decreased by interferon treatment in four interferon-sensitive mouse cell lines which were chronic MLV producers. In three cell lines which were relatively insensitive to interferon, extracellular enzyme activity remained unchanged by interfe...

journal_title：Journal of virology

authors： Friedman RM,Chang EH,Ramseur JM,Myers MW

更新日期：1975-09-01 00:00:00

abstract：:Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and fr...

journal_title：Journal of virology

authors： Matsubara K,Takagi Y,Mukai T

更新日期：1975-09-01 00:00:00

abstract：:Actinophage MSP2 is infectious for Streptomyces venezuelae S13. Based upon electron microscopy of coliphage T4 mixed with MSP2, MSP2 had a head about 48 +/- 2 nm wide and 87 +/- 5 nm long. DNA from polyoma virus and from coliphages T4 and T7 served as reference markers in estimating the molecular weight of MSP2 DNA fr...

journal_title：Journal of virology

authors： Lancaster WD,Jones LA

更新日期：1975-09-01 00:00:00

abstract：:In vitro transcription of the avian tumor virus RNA by RNA-directed DNA polymerase is initiated on the unique cellular 4S RNA. Previous studies have shown that on the average there is one such RNA primer hydrogen bonded to each viral 35S RNA. The present study confirms that finding and demonstrates that, at least for ...

journal_title：Journal of virology

authors： Taylor JM,Illmensee R

更新日期：1975-09-01 00:00:00

abstract：:Ribosomes are observed intimately associated with nucleocapsids of vesicular, stomatitis virus, especially those that line structures that are either cytoplasmic vesicles or invaginations of the plasma membrane. ...

journal_title：Journal of virology

authors： Orenstein J,Shelton E,Lazzarini RA

更新日期：1975-08-01 00:00:00

abstract：:The proteolytic enzyme, thermolysin, degraded the external segment of the membrane glycoprotein of intact vesicular stomatitis (VS) virions but left behind a small nonglycosylated fragment, presumably embedded in the virion membrane. Other proteases generated membrane-associated glycoprotein fragments differing somewh...

journal_title：Journal of virology

authors： Schloemer RH,Wagner RR

更新日期：1975-08-01 00:00:00

abstract：:Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein prepar...

journal_title：Journal of virology

authors： Bishop DH,Repik P,Obijeski JF,Moore NF,Wagner RR

更新日期：1975-07-01 00:00:00

abstract：:The major phosphoprotein common to woolly monkey sarcoma virus, gibbon ape lymphosarcoma virus, and type C viruses of the lower mammalian species (mouse, rat, cat), with the exception of the endogenous cat virus (RD-114), is the polypeptide of about 12,000 molecular weight. The protein-phosphate bond in this polypepti...

journal_title：Journal of virology

authors： Pal BK,McAllister RM,Gardner MB,Roy-Burman P

更新日期：1975-07-01 00:00:00

abstract：:Radioimmunoassays were developed for the 19,000, 15,000, and 12,000 molecular weight polypeptides of avian myeloblastosis virus and for the 19,000 and 12,000 polypeptides of RAV-0, a subgroup E avian tumor virus. Each polypeptide was shown to possess both group- and type-specific antigenic determinants, in contrast to...

journal_title：Journal of virology

authors： Stephenson JR,Smith EJ,Crittenden LB,Aaronson SA

更新日期：1975-07-01 00:00:00

abstract：:The coding properties of RNA transcribed in vitro by purified vaccinia cores have been investigated using Krebs ascites tumor cells, L cells, and reticulocyte lysates. Six to 10 proteins synthesized in vitro are separated on polyacrylamide gels by electrophoresis in the presence of sodium dodecyl sulfate. Their molecu...

journal_title：Journal of virology

authors： Jaureguiberry G,Ben-Hamida F,Chapeville F,Beaud G

更新日期：1975-06-01 00:00:00

abstract：:In search of an anti-transcriptase, antibody was raised in rabbits to partially purified, soluble NS protein present in cytoplasmic extracts of cells infected with the Indiana serotype of vesicular stomatitis (VSInd) virus. This antiserum gave specific reactions of identity by agar immunodiffusion with both cytoplasmi...

journal_title：Journal of virology

authors： Imblum RL,Wagner RR

更新日期：1975-06-01 00:00:00

abstract：:Certain chicken cells that do not spontaneously release virus particles have been shown to produce a subgroup E avian RNA tumor virus, Rous-associated virus 60 (RAV-60), after infection with viruses of other subgroups. The nucleic acids of RAV-60 were analyzed for sequence homologies with the viral nucleic acids conta...

journal_title：Journal of virology

authors： Hayward WS,Hanafusa H

更新日期：1975-06-01 00:00:00

abstract：:The bacteriophage T5 is known to spontaneously generate deletion mutants (st mutants) exhibiting enhanced resistance to heat inactivation in citrate buffer. A series of such mutants has been isolated and the deletions visualized by electron microscopy of heteroduplex molecules. The deletions are found to cluster in on...

journal_title：Journal of virology

authors： Scheible PP,Rhoades M

更新日期：1975-05-01 00:00:00

abstract：:Tryptic peptide maps were prepared for four purified structural proteins derived from several murine leukemia viruses (MuLV's). Analyses of these peptide maps reveal that the p30 proteins of Rauscher, Moloney, and Gross MuLV's are very similar to each other, as are the p10's obtained from these three viruses. In contr...

journal_title：Journal of virology

authors： Buchhagen DL,Stutman O,Fleissner E

更新日期：1975-05-01 00:00:00

abstract：:Nonglucosylated T6 phage (T6gtam 16am30, hereafter called T6alpha gt-) were found to have two structural anomalies when compared with wild-type T6. The DNA of T6alpha gt- phage contains single-strand interruptions. These can be seen both during infection, in the pool of replicating DNA, and in DNA extracted from purif...

journal_title：Journal of virology

authors： Hewlett MJ,Mathews CK

更新日期：1975-04-01 00:00:00

abstract：:Herpesviruses are capable of several types of infection of a host cell. To investigate the early events which ultimately determine the nature of the virus-host cell interaction, a system was established utilizing temperature-sensitive mutants of herpes simplex virus type 2. Four mutants have been isolated which fail t...

journal_title：Journal of virology

authors： Koment RW,Rapp F

更新日期：1975-04-01 00:00:00

abstract：:Defective reovirions lacking the largest (L-1) of the normal 10 genomic segments grow only in association with helper reovirus. Because of the similarity in properties of defective and infectious virions, separation of the two populations by physical methods has been unseccessful. Controlled digestion of purified viru...

journal_title：Journal of virology

authors： Spandidos DA,Graham AF

更新日期：1975-04-01 00:00:00

abstract：:After isolation, the DNA of simian virus 40 appeared as a negative supertwist (form I) or as an open circle with at least one single-strand scission (form II). Under the denaturation conditions usually applied, such as heating in the presence of formaldehyde or application of alkali, form I molecules could appear as "...

journal_title：Journal of virology

authors： Mayer F,Mazaitis AJ,Pühler A

更新日期：1975-03-01 00:00:00

abstract：:The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to...

journal_title：Journal of virology

authors： Melvin P,Kucera LS

更新日期：1975-03-01 00:00:00

abstract：:The replication of vaccinia virus is thought to take place exclusively in the cytoplasm of host cells. However, using DNA-DNA hybridization techniques, it can be shown that a significant fraction of the synthesis of vaccinia DNA takes place in the nucleus as well as the cytoplasm. The (3H) thymiding pulse-labeled vacc...

journal_title：Journal of virology

authors： LaColla P,Weissbach A

更新日期：1975-02-01 00:00:00

abstract：:L2 cells infected with vesicular stomatitis virus under single-cycle conditions have been studied by scanning electron microscopy after preparation by the critical point drying technique. Three dimensional images of intact cells show bullet-shaped vesicular stomatitis virus virions budding singly and in radiating clus...

journal_title：Journal of virology

authors： Holmes KV

更新日期：1975-02-01 00:00:00

abstract：:Mason-Pfizer monkey virus 60-70S RNA has a molecular weight of 8 times 10-6 when analyzed on polyacrylamide gels. Dissociation of 60-70S RNA of Mason-Pfizer monkey virus and murine leukemia virus by heat or formamide (40%) resulted in conversion to identical subunit structures of 2.8 times 10-6 daltons; treatment with...

journal_title：Journal of virology

authors： Schochetman G,Schlom J

更新日期：1975-02-01 00:00:00

abstract：:Synthesis of cytoplasmic viral RNA was studied during infection of cultured human (KB) cells with adenovirus 2. At 6 h, before viral DNA synthesis began 5% of the poly(A)-containing RNA hybridized to viral DNA; by 12 h and at later times more than 80% was virus specified. At 18 h after infection, four major size class...

journal_title：Journal of virology

authors： Tal J,Craig EA,Raskas HJ

更新日期：1975-01-01 00:00:00

abstract：:Type C RNA viruses initially isolated from a lymphosarcoma of a gibbon ape and from a fibrosarcoma of a woolly monkey are very closely related immunologically. However, recent studies have shown that these viruses are distinguishable in a radioimmunoassay for the 12,000-molecular-weight polypeptide (p12) of the woolly...

journal_title：Journal of virology

authors： Tronick SR,Stephenson JR,Aaronson SA,Kawakami TG

更新日期：1975-01-01 00:00:00

abstract：:Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...

journal_title：Journal of virology

authors： Nuss DL,Koch G

更新日期：1975-01-01 00:00:00

abstract：:Myeloblastosis-associated virus (MAV)-2(0), a virus which was derived from avian myeloblastosis virus and induced a high incidence of osteopetrosis, was compared with avian lymphomatosis virus 5938, a recent field isolate which induced a high incidence of lymphomatosis. The following information was obtained. (i) MAV-...

journal_title：Journal of virology

authors： Smith RE,Davids LJ,Neiman PE

更新日期：1975-01-01 00:00:00

abstract：:Purified nuclei from polyoma-infected mouse (3T3) cells were found to be greatly reduced in their ability to synthesize viral DNA in vitro when compared with a crude system consisting of an unfractionated hypotonic lysate of the infected cells. The synthetic capacity of the nuclei could be fully reconstituted when a h...

journal_title：Journal of virology

authors： Francke B,Hunter T

更新日期：1975-01-01 00:00:00

abstract：:Secondary cultures of chicken embryo fibroblasts were infected with the Schmidt Ruppin strain of Rous sarcoma virus (RSV). Five days after infection, the medium was replaced at 2-h intervals with phosphate-free Eagle medium containing 50 muCi of [32P]orthophosphate per ml. Virus was collected by centrifugation, and th...

journal_title：Journal of virology

authors： Jacobson AB,Bromley PA

更新日期：1975-01-01 00:00:00

abstract：:Vaccinia virus DNA fragments that have been denatured by alkali and then neutralized contain a fraction that rapidly reforms duplex structures. The fraction is enriched by fractionating on hydroxyapatite columns and serves as as substrate for digestion by two restriction endonucleases isolated from Hemophilus parainfl...

journal_title：Journal of virology

authors： DeFilippes F

更新日期：1975-01-01 00:00:00

abstract：:The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a ...

journal_title：Journal of virology

authors： Clements JB,Sinsheimer RL

更新日期：1975-01-01 00:00:00

abstract：:The ATP-dependent DNase activity of Escherichia coli disappeared or was markedly reduced after infection with double-stranded DNA phages, T2, T3, T4, T5, T6, T7, lambda, phi80, and P1, but not with the single-stranded DNA phage f1, or the RNA phage Qbeta. This DNase activity was not reduced when chloramphenicol was ad...

journal_title：Journal of virology

authors： Sakaki Y

更新日期：1974-12-01 00:00:00

abstract：:When short pulses of [(3)H]uracil were administered to Bacillus subtilis infected with phage 2C, the main species of labeled RNA was a 10S component that hybridized chiefly, but not exclusively, with the heavy strand of 2C DNA. After long pulses, most of the radioactivity was found in the 23S, 16S, and 5S rRNA's, whic...

journal_title：Journal of virology

authors： Cocito C

更新日期：1974-12-01 00:00:00

abstract：:We have used immunofluorescence in parallel with transmission and scanning electron microscopy to characterize the unusual cytoplasmic and nucleolar accumulation of Simian virus 40 (SV40) virion protein (C antigen) at restrictive temperatures (39 to 41 C) in monkey cells infected with a temperature-sensitive mutant of...

journal_title：Journal of virology

authors： Widmer C,Robb JA

更新日期：1974-12-01 00:00:00

abstract：:Ribosomes from ghost-treated Escherichia coli can form in vitro initiation complexes but cannot translate added template RNA because of a defect in the 30S subunit; 16S rRNA is degraded. ...

journal_title：Journal of virology

authors： Simon M,Kennell D

更新日期：1974-11-01 00:00:00

abstract：:Nine virulent bacteriophages of the anaerobe Corynebacterium (Propionibacterium) acnes, the P-a series, are DNA phages, with long, curved nonretractile tails (130 nm) without tail plates or fibers. They have isometric heads (420 by 460 nm), and are placed in Bradley's group B-1. There is permanent plaque suppression a...

journal_title：Journal of virology

authors： Zierdt CH

更新日期：1974-11-01 00:00:00

abstract：:The isolation of a temperature phage specific for the photosynthetic bacterium Rhodopseudomonas spheroides is reported. This phage, Rphi-1, establishes a state of lysogeny and can be induced from the prophage state by exposure to mitomycin C or UV irradiation. Mutants of Rphi-1 which grow on a standard laboratory stra...

journal_title：Journal of virology

authors： Mural RJ,Friedman DI

更新日期：1974-11-01 00:00:00

abstract：:A tumor line (58-2T) was established from a slowly growing tumor in a BALB/c mouse inoculated with M58-2 cells. The latter clonal cell line was isolated after bromodeoxyuridine treatment as a flat variant from nonproducer BALB/3T3 cells transformed by the Kirsten sarcoma virus. The 58-2T cells produced type C virus wi...

journal_title：Journal of virology

authors： Tsuchida N,Shih MS,Gilden RV,Hatanaka M

更新日期：1974-11-01 00:00:00

abstract：:Influenza virus particles bind rapidly to vesicular stomatitis, Sindbis, or Rauscher murine leukemia virus particles, forming mixed aggregates demonstrable by electron microscopy. The normal hemagglutinating property of influenza virus is inhibited by these viruses, providing a rapid quantitative assay. Prior treatmen...

journal_title：Journal of virology

authors： Compans RW

更新日期：1974-11-01 00:00:00

abstract：:Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intac...

journal_title：Journal of virology

authors： Papas TS,Chirikjian JG,Pry TW,Massicot JG,Irwin RD,Chirigos MA

更新日期：1974-11-01 00:00:00